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Alternatively, the stock lifestyle organisms could possibly be developed in an acceptable liquid medium (i.e., SoybeanCasein Digest Broth or Sabouraud Dextrose Broth) as well as the cells harvested by centrifugation, then washed and resuspended in sterile saline TS to obtain a microbial count of about 1 × 108 cfu per mL. [Be awareThe e